Difference between revisions of "Part:BBa K257023:Experience"

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how you used this part and how it worked out.
 
how you used this part and how it worked out.
  
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===Applications of BBa_K257023===
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===Caracterisation of BBa_K257023===
  
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[[Image:PFec-mRFP1.JPG|Pfec caracterisation]]
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Overnight cultures of BBa_K257023 were diluted 1/100 in LB or M9 medium supplemented with varing amont of FeNO3 and Na Citrate. Fluorescence and OD were assessed in a Tecan plate reader. Fluorescence divided by OD is ploted.
  
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Caracterisation of Fec promoter upon induction with increasing Na Citrate concentrations, on a growth medium with varaiable concentrations of Fe(NO3)3
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[[Image:PFec-mRFP1-LB.JPG|Pfec caracterisation|500px]]
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[[Image:PFec-mRFP1-M9.JPG|Pfec caracterisation|500px]]
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The low Fluo/OD at high FeNO3 concentration can be explained by the taxicity of FeNO3. Note that the best response to Na Citrate is obtained with 1mM FeNO3.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 18:08, 21 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Caracterisation of BBa_K257023

Overnight cultures of BBa_K257023 were diluted 1/100 in LB or M9 medium supplemented with varing amont of FeNO3 and Na Citrate. Fluorescence and OD were assessed in a Tecan plate reader. Fluorescence divided by OD is ploted.

Pfec caracterisation Pfec caracterisation

The low Fluo/OD at high FeNO3 concentration can be explained by the taxicity of FeNO3. Note that the best response to Na Citrate is obtained with 1mM FeNO3.

User Reviews

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