Difference between revisions of "Part:BBa K5115060"
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__TOC__
 
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===Introduction===
 
===Introduction===
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This composite part is composed of [https://parts.igem.org/Part:BBa_K5115065 BBa_K5115065(cso, without csoS3)] and improved ribozyme-assisted polycistronic co-expression system: pRAP.
  
 
===Usage and Biology===
 
===Usage and Biology===
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The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check [https://2022.igem.wiki/fudan/parts part wiki of 2022 Fudan iGEM].
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===Characterization===
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Revision as of 14:22, 28 September 2024


ribozyme+RBS+cso(without csoS3)+stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of BBa_K5115065(cso, without csoS3) and improved ribozyme-assisted polycistronic co-expression system: pRAP.

Usage and Biology

The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check part wiki of 2022 Fudan iGEM.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 208
    Illegal NotI site found at 5126
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 366
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 874
    Illegal AgeI site found at 1825
    Illegal AgeI site found at 2506
    Illegal AgeI site found at 3460
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 266


References