Difference between revisions of "Part:BBa K5115073"
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<partinfo>BBa_K5115073 short</partinfo> | <partinfo>BBa_K5115073 short</partinfo> | ||
| − | <html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM | + | <html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2024"></html> |
__TOC__ | __TOC__ | ||
===Introduction=== | ===Introduction=== | ||
| + | The nikB is a subunit of the nik operon [https://parts.igem.org/Part:BBa_K2652006 BBa_K2652006]. | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | NikB encodes an integral inner membrane protein. It works together with NikC to facilitate the translocation of nickel ions across the inner membrane, contributing to the cell’s ability to import nickel under anaerobic conditions. In our design, NikA is constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115078 BBa_K5115078(ribozyme+RBS+nikB+stem-loop)], which will work alongside the other components of the nikABCDE operon, eventually making up parts [https://parts.igem.org/Part:BBa_K5115082 BBa_K5115082(ribozyme connected nik operon)], which is used for further experiments to facilitate nickel uptake in ''E. coli''. | ||
===Characterization=== | ===Characterization=== | ||
Revision as of 05:06, 25 September 2024
nikB
Introduction
The nikB is a subunit of the nik operon BBa_K2652006.
Usage and Biology
NikB encodes an integral inner membrane protein. It works together with NikC to facilitate the translocation of nickel ions across the inner membrane, contributing to the cell’s ability to import nickel under anaerobic conditions. In our design, NikA is constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115078(ribozyme+RBS+nikB+stem-loop), which will work alongside the other components of the nikABCDE operon, eventually making up parts BBa_K5115082(ribozyme connected nik operon), which is used for further experiments to facilitate nickel uptake in E. coli.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]