Difference between revisions of "Part:BBa K5115035"

Line 6: Line 6:
 
__TOC__
 
__TOC__
 
===Introduction===
 
===Introduction===
 +
This composite part is composed of MTA and improved ribozyme-assisted polycistronic co-expression system: pRAP. The MTA is a protein that can bind with nickel ion and reduce its toxity, get details about MTA in  [https://parts.igem.org/Part:BBa_K5115050 BBa_K5115050(MTA, Metallothionein)].
  
 
===Usage and Biology===
 
===Usage and Biology===
 
+
The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check [https://2022.igem.wiki/fudan/parts part wiki of 2022 Fudan iGEM].
===Characterization===
+
  
  

Revision as of 16:44, 26 September 2024


ribozyme+RBS+MTA+stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of MTA and improved ribozyme-assisted polycistronic co-expression system: pRAP. The MTA is a protein that can bind with nickel ion and reduce its toxity, get details about MTA in BBa_K5115050(MTA, Metallothionein).

Usage and Biology

The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check part wiki of 2022 Fudan iGEM.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 198
  • 1000
    COMPATIBLE WITH RFC[1000]


References