Difference between revisions of "Part:BBa K5115018"
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__TOC__ | __TOC__ | ||
===Introduction=== | ===Introduction=== | ||
| + | This composite part is composed of hypB and improved ribozyme-assisted polycistronic co-expression system: pRAP. The hypB is a subunit of the hydrogenase made up by hypB and hyp, get details about hoxF in [https://parts.igem.org/Part:BBa_K5115020 BBa_K5115020(hox and hyp operon)]. This composite part will finally be used in [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)]and [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067(mineral, F module)]for further experiment. | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check [https://2022.igem.wiki/fudan/parts part wiki of 2022 Fudan iGEM]. | ||
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Revision as of 14:08, 25 September 2024
ribozyme+RBS+hypB+stem-loop
Introduction
This composite part is composed of hypB and improved ribozyme-assisted polycistronic co-expression system: pRAP. The hypB is a subunit of the hydrogenase made up by hypB and hyp, get details about hoxF in BBa_K5115020(hox and hyp operon). This composite part will finally be used in BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module)for further experiment.
Usage and Biology
The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check part wiki of 2022 Fudan iGEM.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 411
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 100
Illegal NgoMIV site found at 946
Illegal AgeI site found at 898 - 1000COMPATIBLE WITH RFC[1000]