Difference between revisions of "Part:BBa K5396005"

 
Line 3: Line 3:
 
<partinfo>BBa_K5396009 short</partinfo>
 
<partinfo>BBa_K5396009 short</partinfo>
  
−
This part is the N-terminal of Spidroin fused to our BaCBM2 with an additional cystein (BBa_)
+
This part is the N-terminal of Spidroin fused to our BaCBM2 with an additional cystein (BBa_K5396003).
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
===Part Generation===
 +
Gibson assembly
  
 
<!-- -->
 
<!-- -->

Revision as of 12:20, 20 September 2024


T7-Nt2RepCt

This part is the N-terminal of Spidroin fused to our BaCBM2 with an additional cystein (BBa_K5396003).

Usage and Biology

Part Generation

Gibson assembly

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 96
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 30
    Illegal XhoI site found at 1148
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 96
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 96
  • 1000
    COMPATIBLE WITH RFC[1000]