Difference between revisions of "Part:BBa K257010"
Line 3: Line 3:
  
 
Insertion of the protein fusion clyA-RFP in the outer membrane of the cell. During vesiculation process, we can observe vesicles by fluorescence.
 
Insertion of the protein fusion clyA-RFP in the outer membrane of the cell. During vesiculation process, we can observe vesicles by fluorescence.
 +
 +
=== Safety Note ===
 +
ClyA is a toxin. For more information see here: <partinfo>BBa_K257002</partinfo>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:18, 22 October 2009

clyA + RFP

Insertion of the protein fusion clyA-RFP in the outer membrane of the cell. During vesiculation process, we can observe vesicles by fluorescence.

Safety Note

ClyA is a toxin. For more information see here: BBa_K257002

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 84
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1494
    Illegal AgeI site found at 1606
  • 1000
    COMPATIBLE WITH RFC[1000]