Difference between revisions of "Part:BBa K5115001"
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2023"></html> | <html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2023"></html> |
Revision as of 22:16, 18 September 2024
hoxF, without stop codon
Introduction
The Ni-Fe hydrogenase we use is an enzyme that can catalyze not only the reduction of hydrogen ions to hydrogen under anaerobic conditions but also the reversed reaction under aerobic conditions. Among this enzyme, hoxF is a hydrogenase subunit responsible for electron transport, which, together with the hoxU subunit, forms the module of NADH dehydrogenase.
Usage and Biology
HoxF subunit is very important in Ni-Fe hydrogenase
The most important group in hoxF is FMN-b, which has the ability of switching electron. Under anaerobic conditions, NADH is oxidized to NAD+ on the surface of hoxF subunit. Under aerobic conditions, NAD+ is reduced to NADH on the surface of the hoxF subunit. Under anaerobic conditions, the electrons generated in this reaction travel through a series of processes to the hoxH, completing the final reduction of the hydrogen ion.
Characterization
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 577
Illegal BglII site found at 1395
Illegal BglII site found at 1688
Illegal XhoI site found at 22 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 456
Illegal NgoMIV site found at 994
Illegal NgoMIV site found at 1204
Illegal NgoMIV site found at 1516 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 625
Illegal BsaI.rc site found at 1129