Difference between revisions of "Part:BBa K5136058"

Revision as of 06:41, 17 September 2024

rhlA

Required for rhamnolipid surfactant production.

===Biology===

The RhlA comes from Pseudomonas aeruginosa and can compete with the enzymes of the type II fatty acid synthase (FASII) cycle for the β-hydroxyacyl-acyl carrier protein (ACP) pathway intermediates, therefore supplies the acyl moieties for rhamnolipid biosynthesis. RhlA has a greater affinity for 10-carbon substrates, and is the only enzyme required to generate the lipid component of rhamnolipid. (1)

===Usage===

We used BBa_K081005 to construct the expression system and obtained the composite BBa_K5136232, which is assembled on the expression vector pSB1C3 by standard assembly. The constructed plasmid was transformed into E. coli DH5α, then the positive transformants were selected by chloramphenicol and confirmed by colony PCR and sequencing.

===Characterization===

==1. Agarose Gel Electrophoresis==

After transferring the plasmid into E. coli DH5α, colony PCR was used to certify the plasmid was correct. The expected bands (bp) was obtained at the position around bp (Fig. 1).

Fig.1 The result of colony PCR products of No part name specified with partinfo tag._pSB1C3.

===Reference===

1. Zhu, K.; Rock, C. O., RhlA converts beta-hydroxyacyl-acyl carrier protein intermediates in fatty acid synthesis to the beta-hydroxydecanoyl-beta-hydroxydecanoate component of rhamnolipids in Pseudomonas aeruginosa. J Bacteriol 2008, 190 (9), 3147-54.

Sequence and Features