Difference between revisions of "Part:BBa K5396004"
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===Usage and Biology===
 
===Usage and Biology===
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<p>The BaCBM2-Cys fragment was generated from a PCR reaction using primers that specifically amplify the linker-BaCBM2-linker region of Bba_BBa_K5396000. The reverse primer used in this reaction adds a codon that encodes the amino acid cysteine at the end of the sequence.</p>
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<p>The BARBIE1-Cys fragment was generated from a PCR reaction using primers that specifically amplify the linker-BARBIE1-linker region of Bba_BBa_K5396001. The reverse primer used in this reaction adds a codon that encodes the amino acid cysteine at the end of the sequence.</p>
  
 
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Revision as of 19:46, 16 September 2024


Barbie1-Cys

BARBIE1 is a synthetic protein derived from BaCBM2 (BBa_K5396003) through a process of reverse engineering. It has the increased ability to bind to plastics when compared to BaCBM2.

This BARBIE1 protein is modified with an additional amino acid (cysteine). This enhancement allows it to be effectively utilized in our biosensor technology.

Usage and Biology

The BARBIE1-Cys fragment was generated from a PCR reaction using primers that specifically amplify the linker-BARBIE1-linker region of Bba_BBa_K5396001. The reverse primer used in this reaction adds a codon that encodes the amino acid cysteine at the end of the sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 91
  • 1000
    COMPATIBLE WITH RFC[1000]