Difference between revisions of "Part:BBa K5492202"
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===Usage and Biology===
 
===Usage and Biology===
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Diamine oxidase (DAO) is an enzyme present in mammals, which plays an important role in digestion, as it is essential for the extracellular metabolism of histamine. DAO oxidizes histamine. The resulting Imidazole-4-acetoldehyde isn’t able to bind to histamine receptors, therefore limiting the biological activity of histamine. Limiting the level of histamine effect can be an appropriate approach in cases of inflammation, where you can anticipate the histamine level increase. On our skin this includes some chronic diseases such as psoriasis, but more often would be useful in handling the acute increase of histamine level, such as a wasp bite. The decrease in oral histamine administration may also help to prevent some acute GI inflammation and smoothing the symptoms of some chronic bowel diseases.
  
 
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Revision as of 20:13, 17 September 2024


Wild type rDAO - pETDUET-1

Wild type coding sequence of rat Diamine Oxidase appropiated for insertion into pT7CFE1.


Usage and Biology

Diamine oxidase (DAO) is an enzyme present in mammals, which plays an important role in digestion, as it is essential for the extracellular metabolism of histamine. DAO oxidizes histamine. The resulting Imidazole-4-acetoldehyde isn’t able to bind to histamine receptors, therefore limiting the biological activity of histamine. Limiting the level of histamine effect can be an appropriate approach in cases of inflammation, where you can anticipate the histamine level increase. On our skin this includes some chronic diseases such as psoriasis, but more often would be useful in handling the acute increase of histamine level, such as a wasp bite. The decrease in oral histamine administration may also help to prevent some acute GI inflammation and smoothing the symptoms of some chronic bowel diseases.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 40
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 40
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 40
    Illegal BglII site found at 1050
    Illegal BglII site found at 2303
    Illegal BamHI site found at 1978
    Illegal BamHI site found at 2232
    Illegal XhoI site found at 848
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 40
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 40
  • 1000
    COMPATIBLE WITH RFC[1000]