Difference between revisions of "Part:BBa K5492402:Design"

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===Design Notes===
 
===Design Notes===
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Restriction sites adapted to pT7CFE1, with adjustions also added for proper framing of the gene.
+
Restriction sites adapted to pETDUET-1, with adjustions also added for proper framing of the gene.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 16:53, 26 September 2024


Wild type HNMT - pETDUET-1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 235
    Illegal XbaI site found at 47
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 235
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 235
    Illegal BglII site found at 1015
    Illegal BamHI site found at 123
    Illegal XhoI site found at 457
    Illegal XhoI site found at 1064
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 235
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 235
    Illegal XbaI site found at 47
    Illegal NgoMIV site found at 1034
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Restriction sites adapted to pETDUET-1, with adjustions also added for proper framing of the gene.

Source

https://www.ncbi.nlm.nih.gov/nuccore/AH003685, BBa_K5492400, BBa_K5492100

References