Difference between revisions of "Part:BBa K5143008"
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| − | ScCBD_cex'V2 : a Cellulose Binding Domain optimised for Saccharomyces cerevisiae, that enables the fixation of proteins to Bacterial Cellulose | + | ScCBD_cex'V2 : a Cellulose Binding Domain optimised for <i>Saccharomyces cerevisiae</i>, that enables the fixation of proteins to Bacterial Cellulose |
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<h1>Description</h1> | <h1>Description</h1> | ||
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<figcaption>Figure 2: Functional cellulose</figcaption> | <figcaption>Figure 2: Functional cellulose</figcaption> | ||
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<img src="https://static.igem.wiki/teams/5143/bba-k5143007-cbd-cexv1-3.png" width="100" alt="ScCBD_Cex coding sequence"> | <img src="https://static.igem.wiki/teams/5143/bba-k5143007-cbd-cexv1-3.png" width="100" alt="ScCBD_Cex coding sequence"> | ||
<figcaption>Figure 3: ScCBD_Cex coding sequence</figcaption> | <figcaption>Figure 3: ScCBD_Cex coding sequence</figcaption> | ||
| + | </figure> | ||
</div> | </div> | ||
<h1>Construction</h1> | <h1>Construction</h1> | ||
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| − | ScCBD-Cex'V2 was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This optimised sequence has been modified to avoid recombination with ScCBD-Cex'V1 in the Yeast. ScCDB-Cex'V2 protein is used in fusion with bioglue composite biobrick (Cp19k_MaSp1) in order to functionalise bacterial cellulose with sticky properties: <a href="https://parts.igem.org/Part:BBa_K5143022" target="_blank">BBa_K5143022</a> | + | ScCBD-Cex'V2 was synthesised and its nucleotide sequence optimised for synthesis and expression in <i>Saccharomyces cerevisiae</i>. This optimised sequence has been modified to avoid recombination with ScCBD-Cex'V1 in the Yeast. ScCDB-Cex'V2 protein is used in fusion with bioglue composite biobrick (Cp19k_MaSp1) in order to functionalise bacterial cellulose with sticky properties: <a href="https://parts.igem.org/Part:BBa_K5143022" target="_blank">BBa_K5143022</a> |
</p> | </p> | ||
<h1>References</h1> | <h1>References</h1> | ||
Revision as of 14:29, 25 September 2024
ScCBD_cex'V2 : a Cellulose Binding Domain, that enables the fixation of proteins to Cellulose
Description
ScCBD_cex'V2 (Cellulose Binding Domain) is a peptide that has a huge affinity for bacterial cellulose. When fused with another protein, ScCBD_cex'V2 enables the fixation of the protein on the bacterial cellulose. This version of ScCBD_Cex has been modified to avoid recombination with ScCBD_Cex'V1 in the yeast.
Construction
ScCBD-Cex'V2 was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This optimised sequence has been modified to avoid recombination with ScCBD-Cex'V1 in the Yeast. ScCDB-Cex'V2 protein is used in fusion with bioglue composite biobrick (Cp19k_MaSp1) in order to functionalise bacterial cellulose with sticky properties: BBa_K5143022
References
Ong, E., Gilkes, N. R., Miller, R. C., Warren, R. A. & Kilburn, D. G. Te cellulose-binding domain (CBD(Cex)) of an exoglucanase from Cellulomonas fmi: production in Escherichia coli and characterization of the polypeptide. Biotechnol. Bioeng. 42, 401–409 (1993). Gilbert, C.; Tang, T.-C.; Ott, W.; Dorr, B. A.; Shaw, W. M.; Sun, G. L.; Lu, T. K.; Ellis, T. Living Materials with Programmable Functionalities Grown from Engineered Microbial Co-Cultures. Nat. Mater. 2021, 20 (5), 691–700. https://doi.org/10.1038/s41563-020-00857-5.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 256
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 226
Illegal AgeI site found at 265 - 1000COMPATIBLE WITH RFC[1000]