Difference between revisions of "Part:BBa K193001"
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This part is used to transform E.coli strain N99,which express cI constitutively, and N4830,which express temperature-sensitive cI constitutively. | This part is used to transform E.coli strain N99,which express cI constitutively, and N4830,which express temperature-sensitive cI constitutively. | ||
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| + | This part is used to transform E.coli strain N99 and N4830, which express cI and temperature-sensitive cI respectively. | ||
We improved existing BioBrick Part F1610(https://parts.igem.org/Part:BBa_F1610), and constructed K193001. | We improved existing BioBrick Part F1610(https://parts.igem.org/Part:BBa_F1610), and constructed K193001. | ||
Revision as of 13:06, 21 October 2009
CI repressible LuxI enzyme generator
cI repressible LuxI enzyme generator.
This part is used to transform E.coli strain N99,which express cI constitutively, and N4830,which express temperature-sensitive cI constitutively.
This part is used to transform E.coli strain N99 and N4830, which express cI and temperature-sensitive cI respectively.
We improved existing BioBrick Part F1610(https://parts.igem.org/Part:BBa_F1610), and constructed K193001.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 712
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]