Difference between revisions of "Part:BBa K5492401:Design"
(→Design Notes) |
(→Design Notes) |
||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | Codon optimisation | + | Codon optimisation for e. coli chassis. |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Revision as of 09:16, 15 September 2024
Optimised Human Histamine-N-Methlytransferase - HNMT
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 100
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 100
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 100
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 100
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 100
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimisation for e. coli chassis.
Source
https://www.ncbi.nlm.nih.gov/nuccore/AH003685, BBa_K5492400
References
Dong H, Nilsson L, Kurland CG. Co-variation of tRNA abundance and codon usage in Escherichia coli at different growth rates. J Mol Biol. 1996 Aug 2;260(5):649-63. doi: 10.1006/jmbi.1996.0428. PMID: 8709146.
Lipinszki Z, Vernyik V, Farago N, Sari T, Puskas LG, Blattner FR, Posfai G, Gyorfy Z. Enhancing the Translational Capacity of E. coli by Resolving the Codon Bias. ACS Synth Biol. 2018 Nov 16;7(11):2656-2664. doi: 10.1021/acssynbio.8b00332. Epub 2018 Nov 2. PMID: 30351909.