Difference between revisions of "Part:BBa K5492401:Design"

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===Source===
 
===Source===
  
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https://www.ncbi.nlm.nih.gov/nuccore/AH003685 , [https://parts.igem.org/Part:BBa_K5492400 BBa_K5492400]
+
https://www.ncbi.nlm.nih.gov/nuccore/AH003685, [https://parts.igem.org/Part:BBa_K5492400 BBa_K5492400]
  
 
===References===
 
===References===

Revision as of 09:04, 15 September 2024


Optimised Human Histamine-N-Methlytransferase - HNMT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 100
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 100
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 100
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 100
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 100
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimisation for the following codons: AGA -> CGT, AGG -> CGT, ATA -> ATC, CCC -> CCG, CGG -> GCT, CTA -> CTG, GGA -> GGC, TCC -> TCT, CTC -> CTG, CCA -> CCG, CTT -> CTG, GGG -> GGC, CCT -> CCG, GCC -> GCT


Source

https://www.ncbi.nlm.nih.gov/nuccore/AH003685, BBa_K5492400

References