Difference between revisions of "Part:BBa K5407000"

Revision as of 08:07, 15 September 2024

pLKO.1 puro plasmid

Usage and Biology

pLKO.1 plasmid is a basic backbone plasmid and can be expressed in mammalian cell lines. The plasmid map can be viewed in the figure 1.The backbone size of the plasmid is 7032 bp,and the selectable markers of the plasmid is puromycin Scientists often use the plasmid tool to inhibit the expression of genes. In our study, we cloned the siRNA sequence targeted to TEAD4 into the basic part pLKO.1 plasmid. And we constructed two composite plasmid named sh-TEAD4-1 and sh-TEAD4-2.Then the TEAD4 mRNA could be degraded in the SW480 cell.

           
                           Figure 1 pLKO.1 map

Sequence and Features