Difference between revisions of "Part:BBa K243004:Design"
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===Design Notes=== | ===Design Notes=== | ||
Glycine and serine are good suitable for repetitive sequences. The properties zwitterionic and hydrophile prevent the aggregation of the linker.<br>The sequence has no cleavage site for proteases. <br> | Glycine and serine are good suitable for repetitive sequences. The properties zwitterionic and hydrophile prevent the aggregation of the linker.<br>The sequence has no cleavage site for proteases. <br> | ||
| − | [[Image:Freiburg09 | + | [[Image:Freiburg09 Shortlipart.png|550x350px]]<br> |
| − | Designed for the fusion of proteins or peptides via in frame cloning, according to [https://parts.igem.org/Assembly_standard_25 RFC 25]. | + | Designed for the fusion of proteins or peptides via in frame cloning, according to [https://parts.igem.org/Assembly_standard_25 RFC 25].<br> |
[https://static.igem.org/mediawiki/parts/e/e6/ShortLinker.txt Commented GenBank file] | [https://static.igem.org/mediawiki/parts/e/e6/ShortLinker.txt Commented GenBank file] | ||
Revision as of 12:54, 21 October 2009
Short Linker (Gly-Gly-Ser-Gly)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Glycine and serine are good suitable for repetitive sequences. The properties zwitterionic and hydrophile prevent the aggregation of the linker.
The sequence has no cleavage site for proteases.
Designed for the fusion of proteins or peptides via in frame cloning, according to RFC 25.
Commented GenBank file
Source
Oligos synthesized by sigma. Hybridized by PCR.