Difference between revisions of "Part:BBa K079032:Experience"

(→‎User Reviews)
Line 1: Line 1:
−
__NOTOC__
 
−
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
−
how you used this part and how it worked out.
 
  
−
===Applications of BBa_K079032===
+
Dh5alpha cells transformed with BBa_K079032 and BBa_K079031 were inoculated
 +
in M9 medium at 8.00 p.m. after O/N growth (about 12 h), samples were collected and slide prepared for image acquisition with the VIFluoR software.
 +
The BBa_K079032/ BBa_K079031 fluorescence ratio was equal to 1.20±0.4 (Table 1).
  
−
===User Reviews===
+
[[Image:TabellaPromotori3.png|center|400px |thumb|Table 1 - Promoter fluorescence ratio after microscope analysis]]
 +
 
 +
The same samples were diluited to an OD equal 0.1 and a growth in time was performed with a Tekcan spectrofluorimeter. Both optical density (OD) and fluorescence level were analized for 12 h (Fig.1 and Fig.2, respectively). Fluorescence was then normalized on the OD value (Fig.3).
 +
 
 +
[[Image:GrowthCurve1.png|center|600px |thumb|Fig.1 - Growth curve]]
 +
[[Image:FluorescenceCurveAbsolute1.png|center|600px |thumb|Fig.2 - Fluorescence]]
 +
[[Image:FluorescenceCurveOverOD1.png|center|600px |thumb|Fig.3 - Fluorescence curve over OD]]
 +
 
 +
As it can be seen from the figures above, data from the fluorimeter analysis agreed with the microscope image analysis. Indeed, the promoter fluorescence ratio was about 1.2.
  
 
<!-- DON'T DELETE --><partinfo>BBa_K079032 StartReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K079032 StartReviews</partinfo>

Revision as of 20:07, 21 October 2009

Dh5alpha cells transformed with BBa_K079032 and BBa_K079031 were inoculated in M9 medium at 8.00 p.m. after O/N growth (about 12 h), samples were collected and slide prepared for image acquisition with the VIFluoR software. The BBa_K079032/ BBa_K079031 fluorescence ratio was equal to 1.20±0.4 (Table 1).

Table 1 - Promoter fluorescence ratio after microscope analysis

The same samples were diluited to an OD equal 0.1 and a growth in time was performed with a Tekcan spectrofluorimeter. Both optical density (OD) and fluorescence level were analized for 12 h (Fig.1 and Fig.2, respectively). Fluorescence was then normalized on the OD value (Fig.3).

Fig.1 - Growth curve
Fig.2 - Fluorescence
Fig.3 - Fluorescence curve over OD

As it can be seen from the figures above, data from the fluorimeter analysis agreed with the microscope image analysis. Indeed, the promoter fluorescence ratio was about 1.2.

UNIQ6f5b9f35cc91d102-partinfo-00000000-QINU UNIQ6f5b9f35cc91d102-partinfo-00000001-QINU