Difference between revisions of "Part:BBa K079031:Experience"

Revision as of 19:10, 21 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K079031

User Reviews

Dh5alpha cells transformed with BBa_K079032 and BBa_K079031 were inoculated in M9 medium at 8.00 p.m. after O/N growth (about 12 h), samples were collected and slide prepared for image acquisition with the VIFluoR software. The BBa_K079032/ BBa_K079031 fluorescence ratio was equal to 1.20±0.4 (Fig.1). The same samples were diluited to an OD equal 0.1 and a growth in time was performed with a Tekcan spectrofluorimeter. Both optical density (OD) and fluorescence level were analized for 12 h (Fig.2 and Fig.3, respectively). Fluorescence was then normalized on the OD value (Fig.4).

Fig.1 - Table of promoter

Fig.2 - Growth curve

Fig.3 - Fluorescence

Fig.4 - Fluorescence curve over OD

UNIQ01ac140384c90a7a-partinfo-00000000-QINU UNIQ01ac140384c90a7a-partinfo-00000001-QINU

@@ Line 6: / Line 6: @@
 ===User Reviews===
+===User Reviews===
+Dh5alpha cells transformed with BBa_K079032 and BBa_K079031 were inoculated
+in M9 medium at 8.00 p.m. after O/N growth (about 12 h), samples were collected and slide prepared for image acquisition with the VIFluoR software.
+The BBa_K079032/ BBa_K079031 fluorescence ratio was equal to 1.20±0.4 (Fig.1).
+The same samples were diluited to an OD equal 0.1 and a growth in time was performed with a Tekcan spectrofluorimeter. Both optical density (OD) and fluorescence level were analized for 12 h (Fig.2 and Fig.3, respectively). Fluorescence was then normalized on the OD value (Fig.4).
+[[Image:TabellaPromotori2.png|center|800px |thumb|Fig.1 - Table of promoter]]
+[[Image:GrowthCurve1.png|center|800px |thumb|Fig.2 - Growth curve]]
+[[Image:FluorescenceCurveAbsolute1.png|center|800px |thumb|Fig.3 - Fluorescence]]
+[[Image:FluorescenceCurveOverOD1.png|center|800px |thumb|Fig.4 - Fluorescence curve over OD]]
 <!-- DON'T DELETE --><partinfo>BBa_K079031 StartReviews</partinfo>
 <!-- Template for a user review