Difference between revisions of "Part:BBa K5061010"

Latest revision as of 22:36, 20 September 2024

PspA promoter

This part is the promoter of the phage shock protein A (pspA) gene of E. coli.

Usage and Biology

The PpspA promoter is actually bidirectional as it contains, in reverse direction, the promoter of the phage shock protein F (pspF) gene of E. coli. This pspA/pspF promoter is induced by stress factors and is regulated by a network of positive and negative mechanisms [Weiner et al., 1991; Elderkin et al., 2002].

References

Weiner L, Brissette JL, Model P. Stress-induced expression of the Escherichia coli phage shock protein operon is dependent on sigma 54 and modulated by positive and negative feedback mechanisms. Genes Dev. 1991 Oct;5(10):1912-23.

Elderkin S, Jones S, Schumacher J, Studholme D, Buck M. Mechanism of action of the Escherichia coli phage shock protein PspA in repression of the AAA family transcription factor PspF. J Mol Biol. 2002 Jun 28;320(1):23-37.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 71
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K5061010 short</partinfo>
-This part is the promoter of the phage shock protein A (pspA) gene of E. coli.
+This part is the promoter of the phage shock protein A (pspA) gene of ''E. coli''.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The PpspA promoter is actually bidirectional as it contains, in reverse direction, the promoter of the phage shock protein F (pspF) gene of ''E. coli''. This pspA/pspF promoter is induced by stress factors and is regulated by a network of positive and negative mechanisms [Weiner et al., 1991; Elderkin et al., 2002].
+===References===
+Weiner L, Brissette JL, Model P. Stress-induced expression of the ''Escherichia coli'' phage shock protein operon is dependent on sigma 54 and modulated by positive and negative feedback mechanisms. Genes Dev. 1991 Oct;5(10):1912-23.
+Elderkin S, Jones S, Schumacher J, Studholme D, Buck M. Mechanism of action of the ''Escherichia coli'' phage shock protein PspA in repression of the AAA family transcription factor PspF. J Mol Biol. 2002 Jun 28;320(1):23-37.
 <!-- -->