Difference between revisions of "Part:BBa K5061007"

 
 
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<partinfo>BBa_K5061007 short</partinfo>
 
<partinfo>BBa_K5061007 short</partinfo>
  
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Golden Gate adapter for Test Plasmid (TP) Golden Gate cloning platform
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This Golden Gate adapter contains two BsaI recognition sites in opposite direction.
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It is present in our composite part [[Part:BBa_K5061034|BBa_K5061034]] and allows rapid engineering using Golden Gate Assembly of Test Plasmid (TP) for phage-assisted continuous and non-continuous evolution (PACE and PANCE).
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 22:12, 20 September 2024


Golden Gate adapter for Test Plasmid (TP) Golden Gate cloning platform

This Golden Gate adapter contains two BsaI recognition sites in opposite direction.

It is present in our composite part BBa_K5061034 and allows rapid engineering using Golden Gate Assembly of Test Plasmid (TP) for phage-assisted continuous and non-continuous evolution (PACE and PANCE).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 18
    Illegal BsaI.rc site found at 6