Difference between revisions of "Part:BBa K5036005"
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==Usage== | ==Usage== | ||
This part is fused with GAL4, dcas9(c). it acts as a powerful transcription activator where it induce transcription and increased gene expression so therefore it increases expression of YAP. | This part is fused with GAL4, dcas9(c). it acts as a powerful transcription activator where it induce transcription and increased gene expression so therefore it increases expression of YAP. | ||
+ | <html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style=" max-width:850px; | ||
+ | width:75%; | ||
+ | height:auto; | ||
+ | position: relative; | ||
+ | top: 50%; | ||
+ | left: 35%; | ||
+ | transform: translate( -50%); | ||
+ | padding-bottom:25px; | ||
+ | padding-top:25px; | ||
+ | "src="https://static.igem.wiki/teams/5036/parts/vp64-attia.png | ||
+ | "> | ||
+ | <p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span | ||
+ | lang=EN style='font-size:11.0pt;line-height:115%'>this figure illustrates the structure and function of VP64 | ||
+ | . </span></p></div></html> | ||
==literature characterization== | ==literature characterization== |
Revision as of 06:39, 12 September 2024
VP64
Part Description
It is a transcriptional activator composed of four tandem copies of VP16 (Herpes Simplex Viral Protein 16, amino acids 437-447*: DALDDFDLDML) connected with glycine-serine (GS) linkers. When fused to another protein domain that can bind near the promoter of a gene, VP64 acts as a strong transcriptional activator
Usage
This part is fused with GAL4, dcas9(c). it acts as a powerful transcription activator where it induce transcription and increased gene expression so therefore it increases expression of YAP.
this figure illustrates the structure and function of VP64 .
literature characterization
vp64 were transfected into HEK293T reporter cells and treated with abscisic acid or DMSO for 48 h.
Statistical significance was calculated with one-way ANOVA, using Dunnett’s multiple testing correction which showed that GFP expression was higher with VP64 than with any other promoter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]