Difference between revisions of "Part:BBa K191003"
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Revision as of 11:39, 21 October 2009
Promoter Lac I - RBS - LovTAP - Term
Part BBa_K191003 codes for the hybrid protein LovTAP. This hybrid protein has a light-sensitive domain ( Lov means light, oxygen, voltage)and a regulatory domain (like Trp repressor). The fused protein allows transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain, therfore results in an increase or decrease of the regulatory domain's affinity for its DNA binding site.
For more details, please consult the section of [http://2009.igem.org/Team:EPF-Lausanne/LOVTAP LovTAP system ] on team EPF-Lausanne wiki.
Usage and Biology
In order to be able to test the efficiency of the fused protein LovTAP, its expression is induced by IPTG ( thanks to the LacI promoter). Two other parts were also constructed as the read out systems of LovTAP : BBa_K191004 and BBa_K191005
All three parts together gives the following system, where LovTAP activities can be measured through RFP fluorescence intensity
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 370
Illegal PstI site found at 627 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 370
Illegal PstI site found at 627 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 370
Illegal PstI site found at 627 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 370
Illegal PstI site found at 627 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 764
Functional Parameters
| input_s | IPTG |
| positive_regulators | -NA- |
| protein_out | LovTAP |