Difference between revisions of "Part:BBa K191003"

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<partinfo>BBa_K191003 short</partinfo>
 
<partinfo>BBa_K191003 short</partinfo>
  
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Part BBa_K191003 codes for the hybrid protein LovTAP. This hybrid protein has a light-sensitive domain ( Lov means light, oxygen, voltage)and a regulatory domain (like Trp repressor). The fused protein allows transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain. The latter would then result in an increase or decrease of the regulatory domain's affinity for its DNA binding site.  
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Part BBa_K191003 codes for the hybrid protein LovTAP. This hybrid protein has a light-sensitive domain ( Lov means light, oxygen, voltage)and a regulatory domain (like Trp repressor). The fused protein allows transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain, therfore results in an increase or decrease of the regulatory domain's affinity for its DNA binding site.  
  
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For more details, please consult the section[http://2009.igem.org/Team:EPF-Lausanne/LOVTAP LovTAP system ] of the EPF-Lausanne wiki.
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 11:22, 21 October 2009

Promoter Lac I - RBS - LovTAP - Term

Part BBa_K191003 codes for the hybrid protein LovTAP. This hybrid protein has a light-sensitive domain ( Lov means light, oxygen, voltage)and a regulatory domain (like Trp repressor). The fused protein allows transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain, therfore results in an increase or decrease of the regulatory domain's affinity for its DNA binding site.

For more details, please consult the section[http://2009.igem.org/Team:EPF-Lausanne/LOVTAP LovTAP system ] of the EPF-Lausanne wiki.

Usage and Biology

In order to be able to test the efficiency of the fused protein LovTAP, its expression is induced by IPTG ( thanks to the LacI promoter). Two other parts were also constructed as the read out systems of LovTAP : BBa_K191004 and BBa_K191005

All three parts together gives the following system, where LovTAP activities can be measured through RFP fluorescence intensity

Systems.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 370
    Illegal PstI site found at 627
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 764