Difference between revisions of "Part:BBa K5101000:Design"
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===Source=== | ===Source=== | ||
| − | The novel antimicrobial peptide is discovered from the <i>Lactobacilli</i> spp. in the human vaginal flora. | + | The novel antimicrobial peptide is discovered from the <i>Lactobacilli</i> spp. in the human vaginal flora. The sequence screening of this protein was done by our host lab of Nanjing Tech University upfront and the related papers and data have not been published yet. |
For reference, the sequence has been identified and is available in the NCBI database under the Reference Sequence: WP_049159833.1. | For reference, the sequence has been identified and is available in the NCBI database under the Reference Sequence: WP_049159833.1. | ||
===References=== | ===References=== | ||
Revision as of 09:34, 10 September 2024
Antimicrobial peptide discovered from the Lactobacillus spp. in the human vaginal flora
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 199
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In order to ensure that the novel antimicrobial peptide of human origin can be successfully expressed in E. coli EcN, we performed codon optimization. Meanwhile, we selected different promoters for gene expression to ensure the feasibility of plasmid design.
Source
The novel antimicrobial peptide is discovered from the Lactobacilli spp. in the human vaginal flora. The sequence screening of this protein was done by our host lab of Nanjing Tech University upfront and the related papers and data have not been published yet. For reference, the sequence has been identified and is available in the NCBI database under the Reference Sequence: WP_049159833.1.