Difference between revisions of "Part:BBa K216016:Experience"

Revision as of 10:17, 21 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K216016

This part is designed to produce a luminescent response in the presence of nitrate or nitrite anions. The bacterial luciferase reaction also requires oxygen and a long chain aldehyde. This can be biosynthesised by the luxCDE gene products (we are preparing a BioBrick with these) or can be supplied in the form of 0.05 - 0.1% v/v n-decanal. Luminescence is blue, around 495 nm (see notes on part BBa_K216008 for more details).

User Reviews

Initial tests: Edinburgh iGEM 2009: This part was tested by growing E. coli JM109/pSB1A2-BBa_K216016 overnight at 37 C with shaking in LB with 80 mg/l ampicillin and varying concentrations of nitrate and nitrite (0, 5, 10 mM sodium nitrite, and 10, 20, 40 mM sodium nitrate).

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 ===User Reviews===
+'''Initial tests: Edinburgh iGEM 2009:''' This part was tested by growing ''E. coli'' JM109/pSB1A2-BBa_K216016 overnight at 37 C with shaking in LB with 80 mg/l ampicillin and varying concentrations of nitrate and nitrite (0, 5, 10 mM sodium nitrite, and 10, 20, 40 mM sodium nitrate).
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