Difference between revisions of "Part:BBa K5049002"
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| + | The sequence of DNA elements for Pir1 anchor protein were from Saccharomyces cerevisiae. To ensure flexibility in connecting to target fusion proteins, a GS linker ((GGSG)3) was added at the N-terminus of Pir1.The GS-Pir1 DNA fragment was synthesized by IDT and cloned into pSB1C3 as a basic part. | ||
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Revision as of 03:41, 10 September 2024
GS-Pir1 (Pir1 anchor protein with a GS linker)
The sequence of DNA elements for Pir1 anchor protein were from Saccharomyces cerevisiae. To ensure flexibility in connecting to target fusion proteins, a GS linker ((GGSG)3) was added at the N-terminus of Pir1.The GS-Pir1 DNA fragment was synthesized by IDT and cloned into pSB1C3 as a basic part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 517 - 1000COMPATIBLE WITH RFC[1000]