Difference between revisions of "Part:BBa K5531006"

 
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pET-Dual-N-His-TEV
 
  
  
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===Usage and Biology===
 
  
 
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===Functional Parameters===
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    <h2>BBa_K5531006 (pET-Dual-N-His-TEV)</h2>
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    <h3>Profile</h3>
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    <p>
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        <strong>Name:</strong> pET-Dual-N-His-TEV<br>
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        <strong>Base Pairs:</strong> 5413 bp<br>
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        <strong>Origin:</strong> <em>Escherichia coli</em>; synthesized<br>
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        <strong>Properties:</strong> A plasmid for efficient co-expression of two target proteins in <em>Escherichia coli</em>, wherein one of the two target proteins expressed can be His-tagged, and a TEV protease cleavage site follows the His-tag. The plasmid contains two polyclonal sites (MCS), each preceded by a T7 promoter/lac operon and a ribosome binding site (RBS), which can efficiently initiate the expression of the target proteins induced by IPTG.
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        <img src="https://static.igem.wiki/teams/5531/bba-k5531006/6.png" alt="Figure 1: Plasmid map of pET-Dual-N-His-TEV">
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        <div class="caption">Fig. 1. Plasmid map of pET-Dual-N-His-TEV</div>
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Revision as of 10:27, 29 September 2024

pET-Dual-N-His-TEV



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NotI site found at 150
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 305
    Illegal BamHI site found at 119
    Illegal XhoI site found at 354
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NgoMIV site found at 324
    Illegal NgoMIV site found at 671
    Illegal NgoMIV site found at 5341
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1256


BBa_K5531006 (pET-Dual-N-His-TEV)

BBa_K5531006 (pET-Dual-N-His-TEV)

Profile

Name: pET-Dual-N-His-TEV
Base Pairs: 5413 bp
Origin: Escherichia coli; synthesized
Properties: A plasmid for efficient co-expression of two target proteins in Escherichia coli, wherein one of the two target proteins expressed can be His-tagged, and a TEV protease cleavage site follows the His-tag. The plasmid contains two polyclonal sites (MCS), each preceded by a T7 promoter/lac operon and a ribosome binding site (RBS), which can efficiently initiate the expression of the target proteins induced by IPTG.

Figure 1: Plasmid map of pET-Dual-N-His-TEV
Fig. 1. Plasmid map of pET-Dual-N-His-TEV