Difference between revisions of "Part:BBa K5117010"
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<partinfo>BBa_K5117010 short</partinfo>
 
<partinfo>BBa_K5117010 short</partinfo>
  
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gene of the uncultured thermophilic bacterium HR29 encoding a polyethylene terephthalate hydrolase (PETase, EC 3.1.1.101), a homologue of the leaf-branch compost cutinase (LCC), already codon optimized for Bacillus subtilis (Xi et al. 2021), but additionally including the signal peptide of the aprE gene for secretion in Bacillus subtilis
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gene of the uncultured thermophilic bacterium HR29 encoding a polyethylene terephthalate hydrolase (PETase, EC 3.1.1.101), a homologue of the leaf-branch compost cutinase (LCC), codon optimized for Bacillus subtilis (Xi et al. 2021), additionally including the signal peptide of the aprE gene for secretion in Bacillus subtilis
  
  

Revision as of 13:59, 31 August 2024


BhrPET

gene of the uncultured thermophilic bacterium HR29 encoding a polyethylene terephthalate hydrolase (PETase, EC 3.1.1.101), a homologue of the leaf-branch compost cutinase (LCC), codon optimized for Bacillus subtilis (Xi et al. 2021), additionally including the signal peptide of the aprE gene for secretion in Bacillus subtilis


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 579
    Illegal AgeI site found at 624
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Xi X., Ni K., Hao H., Shang Y., Zhao B., Qian Z. (2021): Secretory expression in Bacillus subtilis and biochemical characterization of a highly thermostable polyethylene terephthalate hydrolase from bacterium HR29. Enzyme and microbial technology 143, 109715.