Difference between revisions of "Part:BBa K5117010:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K5117010 short</partinfo> | <partinfo>BBa_K5117010 short</partinfo> | ||
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<partinfo>BBa_K5117010 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5117010 SequenceAndFeatures</partinfo> | ||
===Design Notes=== | ===Design Notes=== | ||
removal of restriction sites which are used for RFC10 or TypeIIS standards, addition of a signal peptide SPaprE for secretion in Bacillus subtilis, addition of start codon (ATG) and stop codon (TAA) | removal of restriction sites which are used for RFC10 or TypeIIS standards, addition of a signal peptide SPaprE for secretion in Bacillus subtilis, addition of start codon (ATG) and stop codon (TAA) | ||
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===Source=== | ===Source=== | ||
Bacterium HR29 | Bacterium HR29 | ||
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===References=== | ===References=== | ||
Xi X., Ni K., Hao H., Shang Y., Zhao B., Qian Z. (2021): Secretory expression in Bacillus subtilis and biochemical characterization of a highly thermostable polyethylene terephthalate hydrolase from bacterium HR29. Enzyme and microbial technology 143, 109715. | Xi X., Ni K., Hao H., Shang Y., Zhao B., Qian Z. (2021): Secretory expression in Bacillus subtilis and biochemical characterization of a highly thermostable polyethylene terephthalate hydrolase from bacterium HR29. Enzyme and microbial technology 143, 109715. | ||
Revision as of 12:00, 31 August 2024
BhrPET
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 579
Illegal AgeI site found at 624 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
removal of restriction sites which are used for RFC10 or TypeIIS standards, addition of a signal peptide SPaprE for secretion in Bacillus subtilis, addition of start codon (ATG) and stop codon (TAA)
Source
Bacterium HR29
References
Xi X., Ni K., Hao H., Shang Y., Zhao B., Qian Z. (2021): Secretory expression in Bacillus subtilis and biochemical characterization of a highly thermostable polyethylene terephthalate hydrolase from bacterium HR29. Enzyme and microbial technology 143, 109715.