Difference between revisions of "Part:BBa K5117010"
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gene of the uncultured thermophilic bacterium HR29 encoding a polyethylene terephthalate hydrolase (PETase), a homologue of the leaf-branch compost cutinase (LCC), already codon optimized for Bacillus subtilis (Xi et al. 2021), but additionally including the signal peptide of the aprE gene for secretion in Bacillus subtilis | gene of the uncultured thermophilic bacterium HR29 encoding a polyethylene terephthalate hydrolase (PETase), a homologue of the leaf-branch compost cutinase (LCC), already codon optimized for Bacillus subtilis (Xi et al. 2021), but additionally including the signal peptide of the aprE gene for secretion in Bacillus subtilis | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K5117010 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5117010 SequenceAndFeatures</partinfo> | ||
Revision as of 11:58, 31 August 2024
BhrPET
gene of the uncultured thermophilic bacterium HR29 encoding a polyethylene terephthalate hydrolase (PETase), a homologue of the leaf-branch compost cutinase (LCC), already codon optimized for Bacillus subtilis (Xi et al. 2021), but additionally including the signal peptide of the aprE gene for secretion in Bacillus subtilis
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 579
Illegal AgeI site found at 624 - 1000COMPATIBLE WITH RFC[1000]
References
Xi X., Ni K., Hao H., Shang Y., Zhao B., Qian Z. (2021): Secretory expression in Bacillus subtilis and biochemical characterization of a highly thermostable polyethylene terephthalate hydrolase from bacterium HR29. Enzyme and microbial technology 143, 109715.