Difference between revisions of "Part:BBa K283003"

Revision as of 09:15, 21 October 2009

pTet-rbs-chez_histag-double terminator

This device is an improved version of BBa_K094102. It was designed to express CheZ protein, which is responsible for the dephosphorylation of CheY, a major effector in bacterial flagella rotation. Since lacking an antibody to recognize the expressed CheZ, we fused a His6-tag to the cheZ encoding region. cheZ gene was then put under the control of the pTet promoter, whose expression can be induced by adding anhydrotetracycline (aTc). The backbone of this biobrick is BBa_J61003. The strong double terminators lie between XbaI and SpeI sites.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 726
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K283003 short</partinfo>
-This device is an improved version of BBa_K094102. It was designed to express the CheZ, which is responsible for the dephosphorylation of CheY involved in bacterial flagella rotation. Since lacking an antibody to recognize the expressed CheZ, we fused a His6-tag to the ''cheZ'' encoding region. We put'' cheZ'' gene under the control of the pTet promoter, which is repressed by the TetR repressor protein(C0040) and can be induced by adding anhydrotetracycline (aTc). The backbone of this biobrick is BBa_J61003. The strong double terminators lie between ''Xba''I and ''Spe''I sites.
+This device is an improved version of BBa_K094102. It was designed to express CheZ protein, which is responsible for the dephosphorylation of CheY, a major effector in bacterial flagella rotation. Since lacking an antibody to recognize the expressed CheZ, we fused a His6-tag to the ''cheZ'' encoding region. '' cheZ'' gene was then put under the control of the pTet promoter, whose expression can be induced by adding anhydrotetracycline (aTc). The backbone of this biobrick is BBa_J61003. The strong double terminators lie between ''Xba''I and ''Spe''I sites.