Difference between revisions of "Part:BBa K283002"

Revision as of 08:52, 21 October 2009

Speed control device (pLacI-rbs-TetR-terminator-ptet-rbs-chez_his-double terminators)

To test if cheZ expression level was proportional to the bacterial swimming speed and hopefuly to control the speed, we designed and made this speed controller device. The full length cheZ gene fused with His6-tag was put under the control of pTet, whose expression is repressed by constitutively expressed TetR and can be induced by the addition of anhydrotetracycline or tetracycline. Since lacking antibody to recognize the CheZ protein, His6-tag was added to facilitate the measurement. This device has been demonstrated to work as expected in our system (see experience or visit our wiki).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1782
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 07:53, 21 October 2009 (view source) HKUHKBU (Talk \| contribs) ← Older edit		Revision as of 08:52, 21 October 2009 (view source) HKUHKBU (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K283002 short</partinfo>		<partinfo>BBa_K283002 short</partinfo>

−	To test if ''cheZ'' expression level was proportional to the bacterial swimming speed and hopefuly to control the speed, we designed and made this speed controller device. The full length ''cheZ'' gene fused with His6-tag was put under the control of pTet, whose expression is repressed by constitutively expressed TetR and can be induced by the addition of anhydrotetracycline or tetracycline. Since lacking antibody to recognize the CheZ protein, His6-tag was added to facilitate the measurement. This device has been demonstrated to work in our system (see experience or visit our wiki).	+	To test if ''cheZ'' expression level was proportional to the bacterial swimming speed and hopefuly to control the speed, we designed and made this speed controller device. The full length ''cheZ'' gene fused with His6-tag was put under the control of pTet, whose expression is repressed by constitutively expressed TetR and can be induced by the addition of anhydrotetracycline or tetracycline. Since lacking antibody to recognize the CheZ protein, His6-tag was added to facilitate the measurement. This device has been demonstrated to work as expected in our system (see experience or visit our wiki).