Difference between revisions of "Part:BBa K5108004"

 
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
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<ol style="color: black; padding: auto -1rem; margin= 0"> <b>Contents</b>
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    <li style="color: blue;">Usage and Biology</li>
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    <li style="color: blue;">References</li>
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<h2 style="color: blue;"> <b>Usage and Biology</b></h2>
  
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<p><a href="https://www.uniprot.org/uniprotkb/P83772/entry" target="blank">CrnA</a> is an enzyme that catalyzes the degradation of creatinine into creatine in <i>Pseudomonas putida</i>.
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===Functional Parameters===
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In the context of our project, the gene encoding this enzyme (<i>crnA</i>) was synthesized in an operon with the gene for creatine degradation. You can find more information about our construct and our results in <a href="https://parts.igem.org/Part:BBa_K5108009" target="blank">BBa_K5108009</a>.
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<h2 style="color: blue;"><b>References</b></h2>
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<li>UniProt. (s. d.). https://www.uniprot.org/uniprotkb/P83772/entry</li>
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<li>Tsuru, D., Oka, I., & Yoshimoto, T. (1976c). Creatinine Decomposing Enzymes inPseudomonas putida. Agricultural And Biological Chemistry, 40(5), 1011‑1018. https://doi.org/10.1080/00021369.1976.10862151</li>
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Revision as of 12:54, 29 September 2024

Creatinine amidohydrolase form Pseudomonas putida


    Contents
  1. Usage and Biology
  2. References



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 61
    Illegal NgoMIV site found at 663
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 199
    Illegal BsaI.rc site found at 547

Usage and Biology

CrnA is an enzyme that catalyzes the degradation of creatinine into creatine in Pseudomonas putida. In the context of our project, the gene encoding this enzyme (crnA) was synthesized in an operon with the gene for creatine degradation. You can find more information about our construct and our results in BBa_K5108009.


References

  1. UniProt. (s. d.). https://www.uniprot.org/uniprotkb/P83772/entry
  2. Tsuru, D., Oka, I., & Yoshimoto, T. (1976c). Creatinine Decomposing Enzymes inPseudomonas putida. Agricultural And Biological Chemistry, 40(5), 1011‑1018. https://doi.org/10.1080/00021369.1976.10862151
    3.