Difference between revisions of "Part:BBa K5198105"

Latest revision as of 10:57, 2 October 2024

IFN-NanoLuc
NanoLuciferase (BBa_K1734004 ) with IFN tag (BBa_K4646001 ) . This part was used in solubility studies to find the optimal secretion signal peptide for neoantigens in ATLAS (Figure 1).

Figure 1. Chemiluminescence readings of NL with different signal peptide fusions in HEK293T cell supernatant. The negative control (NC) is NL fused with an HA tag, which does not signal extracellular secretion. Error bars represent the standard deviation (SD) of 4 biological replicates. The data was normalized by subtracting background signal.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 527

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K5198105 short</partinfo>
+<html>
+<br>
+NanoLuciferase (<a href="https://parts.igem.org/Part:BBa_K1734004">BBa_K1734004</a>
+) with IFN tag (<a href="https://parts.igem.org/Part:BBa_K4646001">BBa_K4646001</a>
+) . This part was used in solubility studies to find the optimal secretion signal peptide for neoantigens in ATLAS (<b>Figure 1</b>).
+<div style="text-align: center;">
+<img src="https://static.igem.wiki/teams/5198/results/figure-4v3.webp" alt="Description of the image" style="width: 400px; height: auto;">
+</div>
+<p><b>Figure 1.</b> Chemiluminescence readings of NL with different signal peptide fusions in HEK293T cell supernatant. The negative control (NC) is NL fused with an HA tag, which does not signal extracellular secretion. Error bars represent the standard deviation (SD) of 4 biological replicates. The data was normalized by subtracting background signal.</p>
+</html>
-NanoLuciferase with IFN tag. This part was used in solubility studies to find the optimal secretion signal peptide for neoantigens in ATLAS.
 <!-- Add more about the biology of this part here