Difference between revisions of "Part:BBa K5198103"

 
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<partinfo>BBa_K5198103 short</partinfo>
 
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NanoLuciferase with HA tag. The part can be used as a negative control in secretion studies as HA tag does not signal extracellular export of the protein.
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This part is composed of nanoluciferase (<a href="https://parts.igem.org/Part:BBa_K1734004">BBa_K1734004</a>
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) with the human influenza hemagglutinin (HA) tag (<a href="https://parts.igem.org/Part:BBa_K1150016">BBa_K1150016</a>
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).
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<p>The part can be used as a null control in secretion studies as the HA tag does not signal extracellular export of the protein (<b>Figure 1</b>).</p>
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<div style="text-align: center;">
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<img src="https://static.igem.wiki/teams/5198/results/figure-3v3.webp" alt="Description of the image" style="width: 400px; height: auto;">
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</div>
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<p><b>Figure 1.</b> Chemiluminescence readings of NL with different signal peptide fusions in HEK293T cell supernatant. The negative control (NC) is NL fused with an HA tag, which does not signal extracellular secretion. Error bars represent the standard deviation (SD) of 4 biological replicates. The data was normalized by subtracting background signal.</p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:29, 2 October 2024


HA-NanoLuc This part is composed of nanoluciferase (BBa_K1734004 ) with the human influenza hemagglutinin (HA) tag (BBa_K1150016 ).

The part can be used as a null control in secretion studies as the HA tag does not signal extracellular export of the protein (Figure 1).

Description of the image

Figure 1. Chemiluminescence readings of NL with different signal peptide fusions in HEK293T cell supernatant. The negative control (NC) is NL fused with an HA tag, which does not signal extracellular secretion. Error bars represent the standard deviation (SD) of 4 biological replicates. The data was normalized by subtracting background signal.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 485