Difference between revisions of "Part:BBa K5198011:Design"
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===References=== | ===References=== | ||
| + | “Commonly Used Leader Peptide Sequences for Mammalian Cells Expression.” NovoPro 21 Apr. 2018, https://www.novoprolabs.com/support/articles/commonly-used-leader-peptide-sequences-for-efficient-secretion-of-a-recombinant-protein-expressed-in-mammalian-cells-201804211337.html. | ||
Latest revision as of 15:40, 23 August 2024
CTR tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence needs to be added to N-terminus of the protein cargo to secrete properly.
Source
The amino acid sequence was reverse transcribed and codon optimized for expression in mammalian cells.
References
“Commonly Used Leader Peptide Sequences for Mammalian Cells Expression.” NovoPro 21 Apr. 2018, https://www.novoprolabs.com/support/articles/commonly-used-leader-peptide-sequences-for-efficient-secretion-of-a-recombinant-protein-expressed-in-mammalian-cells-201804211337.html.