Difference between revisions of "Part:BBa K5198000:Design"
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===Source=== | ===Source=== | ||
| − | The sequence was created by reverse transcription of human CD19 protein (UniProt: P15391). The ectodomain was codon optimized for expression in human cells. | + | The sequence was created by reverse transcription of human CD19 protein (UniProt: P15391). The ectodomain was codon optimized for expression in human cells. |
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Latest revision as of 07:35, 25 August 2024
CD19ecto
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 225
Illegal SapI.rc site found at 61
Design Notes
The sequence contains native CD19 signal tag, but will secrete due to the lack of transmembrane domain. The ectodomain sequence was determined based on the annotation in UniProt entry.
Source
The sequence was created by reverse transcription of human CD19 protein (UniProt: P15391). The ectodomain was codon optimized for expression in human cells.