Difference between revisions of "Part:BBa K5115001"

 
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2023"></html>
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===Introduction===
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The Ni-Fe hydrogenase we use is an enzyme that can catalyze not only the reduction of hydrogen ions to hydrogen under anaerobic conditions but also the reversed reaction under aerobic conditions. Among this enzyme, hoxF is a hydrogenase subunit responsible for electron transport, which, together with the hoxU subunit, forms the module of NADH dehydrogenase.
  
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Microbe sample from Cupriavidus necator H16.
 
  
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===Usage and Biology===
 
===Usage and Biology===
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HoxF subunit is very important in Ni-Fe hydrogenase
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The most important group in hoxF is FMN-b, which has the ability of switching electron. Under anaerobic conditions, NADH is oxidized to NAD<sup>+</sup> on the surface of hoxF subunit. Under aerobic conditions, NAD<sup>+</sup> is reduced to NADH on the surface of the hoxF subunit. Under anaerobic conditions, the electrons generated in this reaction travel through a series of processes to the hoxH, completing the final reduction of the hydrogen ion.
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===Characterization===
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<span class='h3bb'>Sequence and Features</span>
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K5115001 parameters</partinfo>
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<partinfo>BBa_K5115000 parameters</partinfo>
 
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==References==
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<references />

Revision as of 06:48, 17 September 2024


RcnR_C35L

contributed by Fudan iGEM 2023

Introduction

The Ni-Fe hydrogenase we use is an enzyme that can catalyze not only the reduction of hydrogen ions to hydrogen under anaerobic conditions but also the reversed reaction under aerobic conditions. Among this enzyme, hoxF is a hydrogenase subunit responsible for electron transport, which, together with the hoxU subunit, forms the module of NADH dehydrogenase.


Usage and Biology

HoxF subunit is very important in Ni-Fe hydrogenase

The most important group in hoxF is FMN-b, which has the ability of switching electron. Under anaerobic conditions, NADH is oxidized to NAD+ on the surface of hoxF subunit. Under aerobic conditions, NAD+ is reduced to NADH on the surface of the hoxF subunit. Under anaerobic conditions, the electrons generated in this reaction travel through a series of processes to the hoxH, completing the final reduction of the hydrogen ion.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 228
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References