Difference between revisions of "Part:BBa K5267011"

 
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A signal peptide is a hydrophobic amino acid sequence located at the N-terminus of a protein encoding a length of 5-30 amino acids used to direct newly synthesised proteins towards the endoplasmic reticulum. Signal peptides are found in secreted proteins, transmembrane proteins, and proteins within eukaryotic organelles.
 
  
 
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===Profile===
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Name: mPGK promoter
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<br>Base Pairs: 516bp
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<br>Origin: Mouse
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<br>Properties: promoter that responsible for transcription of MTNR1a
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===Usage and Biology===
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MPGK promoter(mpPGK) is the promoter of Pgk1 in mouse cell. Pgk-1 is the murine gene encoding phosphoglycerate kinase (PGK), a key enzyme in glycolysis. This enzyme must  be present in all cells, so the Pgk-1 promoter is expected to be a ubiquitously active one<sup>[1]</sup>.
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The cloned mpPGK is very active in driving transcription of reporter genes following transfection into mammalian cells<sup>[2]</sup>. The promoter is particularly active in pluripotent embryonal carcinoma and embryonic stem cells.
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<br>An initial studie on the Pgk-1 promoter indicated that its activity was contained within a region from −425 bp to +80 bp relative to the first transcription start site[2]. This promoter is GC rich and contains no TATA box<sup>[3]</sup>. The data from John P. Thomson et al. indicate that a primary function of non-methylated CGIs is to genetically influence the local chromatin modification state to initiate transcriptionby interaction with Cfp1 and perhaps other CpG-binding proteins<sup>[4]</sup>. The core promoter from −121 bp to +80 bp contains a number of putative Spl binding sites and had modest promoter activity. The 304 bp upstream of the core promoter had enhancer-like activity such that this region increased expression from the core promoter in an orientation- and position-independent fashion.

Revision as of 08:50, 22 September 2024


mPGK Promoter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 116
  • 1000
    COMPATIBLE WITH RFC[1000]


Profile

Name: mPGK promoter
Base Pairs: 516bp
Origin: Mouse
Properties: promoter that responsible for transcription of MTNR1a


Usage and Biology

MPGK promoter(mpPGK) is the promoter of Pgk1 in mouse cell. Pgk-1 is the murine gene encoding phosphoglycerate kinase (PGK), a key enzyme in glycolysis. This enzyme must be present in all cells, so the Pgk-1 promoter is expected to be a ubiquitously active one[1]. The cloned mpPGK is very active in driving transcription of reporter genes following transfection into mammalian cells[2]. The promoter is particularly active in pluripotent embryonal carcinoma and embryonic stem cells.
An initial studie on the Pgk-1 promoter indicated that its activity was contained within a region from −425 bp to +80 bp relative to the first transcription start site[2]. This promoter is GC rich and contains no TATA box[3]. The data from John P. Thomson et al. indicate that a primary function of non-methylated CGIs is to genetically influence the local chromatin modification state to initiate transcriptionby interaction with Cfp1 and perhaps other CpG-binding proteins[4]. The core promoter from −121 bp to +80 bp contains a number of putative Spl binding sites and had modest promoter activity. The 304 bp upstream of the core promoter had enhancer-like activity such that this region increased expression from the core promoter in an orientation- and position-independent fashion.