Difference between revisions of "Part:BBa K5071007"

 
 
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BGCII-2
 
  
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===Usage and Biology===
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===Functional Parameters===
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    <h2>Composite part BBa_K5071007 (BGCII-2)</h2>
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    <h3>Name: BGCII-2</h3>
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    <p><strong>Base Pairs:</strong> 2000 bp</p>
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    <p><strong>Origin:</strong> Paceibacterales</p>
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    <h3>Usage and Biology</h3>
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        BGCII-2 is a gene identified within a predicted terpene biosynthesis gene cluster, with bioinformatic tools suggesting a potential role in terpene production. Although its precise function remains unknown, sequence analysis indicates the presence of conserved domains common to enzymes involved in secondary metabolite pathways, highlighting its potential importance in the biosynthesis of terpenoids. Further experimental validation is required to elucidate its specific role.
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    <h3>Cultivation</h3>
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        We used PCR to amplify the BGCII-2 gene, with a length of 2000 bp. Fig 1 shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid pETDuet-BGCII-gene792.
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        <img src="https://static.igem.wiki/teams/5071/bba-k5071007/1.jpg" alt="Fig 1. The purpose segment of plasmid pACYCDuet-BGCII-gene792">
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        <div class="caption">Fig 1. The purpose segment of plasmid pACYCDuet-BGCII-gene792</div>
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Latest revision as of 04:46, 30 September 2024


BGCII-2



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 937
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 937
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 621
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 937
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 937
    Illegal NgoMIV site found at 1362
    Illegal NgoMIV site found at 1848
    Illegal AgeI site found at 1036
  • 1000
    COMPATIBLE WITH RFC[1000]


<!DOCTYPE html> BBa_K5071007 (BGCII-2)

Composite part BBa_K5071007 (BGCII-2)

Name: BGCII-2

Base Pairs: 2000 bp

Origin: Paceibacterales

Usage and Biology

BGCII-2 is a gene identified within a predicted terpene biosynthesis gene cluster, with bioinformatic tools suggesting a potential role in terpene production. Although its precise function remains unknown, sequence analysis indicates the presence of conserved domains common to enzymes involved in secondary metabolite pathways, highlighting its potential importance in the biosynthesis of terpenoids. Further experimental validation is required to elucidate its specific role.

Cultivation

We used PCR to amplify the BGCII-2 gene, with a length of 2000 bp. Fig 1 shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid pETDuet-BGCII-gene792.

Fig 1. The purpose segment of plasmid pACYCDuet-BGCII-gene792
Fig 1. The purpose segment of plasmid pACYCDuet-BGCII-gene792