Difference between revisions of "Part:BBa K5071001"

 
 
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BGCI-2
 
  
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===Usage and Biology===
 
  
 
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===Functional Parameters===
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    <h2>Composite part BBa_K5071001 (BGCI-2)</h2>
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    <h3>Name: BGCI-2</h3>
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    <p><strong>Base Pairs:</strong> 1200 bp</p>
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    <p><strong>Origin:</strong> Bacteriovoracaceae</p>
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    <h3>Usage and Biology</h3>
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        BGCI-2 is a gene identified within a predicted terpene biosynthesis gene cluster, with bioinformatic tools suggesting a potential role in terpene production. Although its precise function remains unknown, sequence analysis indicates the presence of conserved domains common to enzymes involved in secondary metabolite pathways, highlighting its potential importance in the biosynthesis of terpenoids. Further experimental validation is required to elucidate its specific role.
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    <h3>Cultivation</h3>
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        We used PCR to amplify the BGCI-2 gene, with a length of 1200 bp. Fig 1 shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid pETDuet-BGCI-gene123.
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        <img src="https://static.igem.wiki/teams/5071/bba-k5071001/1.jpg" alt="Fig 1. The purpose segment of plasmid pETDuet-BGC1-gene123">
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        <div class="caption">Fig 1. The purpose segment of plasmid pETDuet-BGC1-gene123</div>
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Latest revision as of 04:35, 30 September 2024


BGCI-2


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 132
    Illegal PstI site found at 349
    Illegal PstI site found at 439
    Illegal PstI site found at 759
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 132
    Illegal PstI site found at 349
    Illegal PstI site found at 439
    Illegal PstI site found at 759
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 132
    Illegal PstI site found at 349
    Illegal PstI site found at 439
    Illegal PstI site found at 759
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 132
    Illegal PstI site found at 349
    Illegal PstI site found at 439
    Illegal PstI site found at 759
    Illegal AgeI site found at 1039
  • 1000
    COMPATIBLE WITH RFC[1000]


BBa_K5071001 (BGCI-2)

Composite part BBa_K5071001 (BGCI-2)

Name: BGCI-2

Base Pairs: 1200 bp

Origin: Bacteriovoracaceae

Usage and Biology

BGCI-2 is a gene identified within a predicted terpene biosynthesis gene cluster, with bioinformatic tools suggesting a potential role in terpene production. Although its precise function remains unknown, sequence analysis indicates the presence of conserved domains common to enzymes involved in secondary metabolite pathways, highlighting its potential importance in the biosynthesis of terpenoids. Further experimental validation is required to elucidate its specific role.

Cultivation

We used PCR to amplify the BGCI-2 gene, with a length of 1200 bp. Fig 1 shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid pETDuet-BGCI-gene123.

Fig 1. The purpose segment of plasmid pETDuet-BGC1-gene123
Fig 1. The purpose segment of plasmid pETDuet-BGC1-gene123