Difference between revisions of "Part:BBa K5071000"

 
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BGCI-1
 
  
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===Usage and Biology===
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===Functional Parameters===
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        <h2>BBa_K5071000 (BGCI-1)</h2>
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        <h3>Name: BGCI-1</h3>
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        <p><strong>Base Pairs:</strong> 150 bp</p>
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        <p><strong>Origin:</strong> Bacteriovoracaceae</p>
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        <h3>Usage and Biology</h3>
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            BGCI-1 is a gene identified within a predicted terpene biosynthesis gene cluster, with bioinformatic tools suggesting a potential role in terpene production. Although its precise function remains unknown, sequence analysis indicates the presence of conserved domains common to enzymes involved in secondary metabolite pathways, highlighting its potential importance in the biosynthesis of terpenoids. Further experimental validation is required to elucidate its specific role.
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        <h3>Cultivation</h3>
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            We used PCR to amplify the BGCI-1 gene, with a length of 150 bp. Fig 1 shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid pETDuet-BGCI-gene123.
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        <img src="https://static.igem.wiki/teams/5071/bba-k5071000/1.jpg" alt="Fig 1. The purpose segment of plasmid pETDuet-BGC1-gene123">
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        <div class="caption">Fig 1. The purpose segment of plasmid pETDuet-BGC1-gene123</div>
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Revision as of 04:29, 30 September 2024

BGCI-1



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


<!DOCTYPE html> BBa_K5071000 (BGCI-1)

BBa_K5071000 (BGCI-1)

Name: BGCI-1

Base Pairs: 150 bp

Origin: Bacteriovoracaceae

Usage and Biology

BGCI-1 is a gene identified within a predicted terpene biosynthesis gene cluster, with bioinformatic tools suggesting a potential role in terpene production. Although its precise function remains unknown, sequence analysis indicates the presence of conserved domains common to enzymes involved in secondary metabolite pathways, highlighting its potential importance in the biosynthesis of terpenoids. Further experimental validation is required to elucidate its specific role.

Cultivation

We used PCR to amplify the BGCI-1 gene, with a length of 150 bp. Fig 1 shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid pETDuet-BGCI-gene123.

Fig 1. The purpose segment of plasmid pETDuet-BGC1-gene123
Fig 1. The purpose segment of plasmid pETDuet-BGC1-gene123