Difference between revisions of "Part:BBa K5291006:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The acaP is from Rhodopseudomonas palustris CGA009, which ensures its normal expression in the engineered R.palistris. | + | The gene <i>acaP</i> is from <i>Rhodopseudomonas palustris</i> CGA009, which ensures its normal expression in the engineered <i>R.palistris</i>. |
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===Source=== | ===Source=== | ||
Revision as of 03:15, 28 September 2024
acaP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 223
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 223
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 152
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 223
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 223
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene acaP is from Rhodopseudomonas palustris CGA009, which ensures its normal expression in the engineered R.palistris.
Source
Rhodopseudomonas palustris CGA009
References
[1]Puskas, L.G., et al., A periplasmic, alpha-type carbonic anhydrase from Rhodopseudomonas palustris is essential for bicarbonate uptake. Microbiology (Reading), 2000. 146 ( Pt 11): p. 2957-2966. [2]Thorbecke, R., et al., The gene dosage effect of carbonic anhydrase on the biosynthesis of poly ( 3-hydroxybutyrate ) under autotrophic and mixotrophic culture conditions. POLYMER JOURNAL, 2021. 53(1): p. 209-213.