Difference between revisions of "Part:BBa K5291006"

Revision as of 05:20, 11 August 2024

acaP

The gene acaP comes from Rhodopseudomonas palustris CGA009, and it can encode a kind of α-carbonic anhydrase that is able to speed up the reversible conversion between CO2 and bicarbonate. In our design, engineered Pseudomonas aeruginosa PAO1 converse CO2 into bicarbonate, in order to deliver the carbon source in the biofilm more easily. Rhodopseudomonas palustris CGA009 take in the bicarbonate and transform it as CO2 for CBB cycle. The efficiency of the conversion in Rhodopseudomonas palustris WT is limited. Therefore, we introduce acaP into Rhodopseudomonas palustris CGA009 to realize overexpressing of the carbonic anhydrase, which enable the engineered Rhodopseudomonas palustris to converse bicarbonate into CO2 in a higher rate, accelerating the process of carbon fixation in Rhodopseudomonas palustris CGA009.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 223
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 223
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 152
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 223
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 223
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K5291006 short</partinfo>
-The gene comes from Rhodopseudomonas palustris CGA009, and it can encode a kind of &#945;-carbonic anhydrase that is able to speed up the reversible conversion between CO2 and bicarbonate. In our design, engineered Pseudomonas aeruginosa PAO1 converse CO2 into bicarbonate, in order to deliver the carbon source in the biofilm more easily. Rhodopseudomonas palustris CGA009 take in the bicarbonate and transform it as CO2 for CBB cycle. The efficiency of the conversion in Rhodopseudomonas palustris WT is limited. Therefore, we introduce acaP into Rhodopseudomonas palustris CGA009 to realize overexpressing of the carbonic anhydrase, which enable the engineered Rhodopseudomonas palustris to converse bicarbonate into CO2 in a higher rate, accelerating the process of carbon fixation in Rhodopseudomonas palustris CGA009.
+The gene acaP comes from Rhodopseudomonas palustris CGA009, and it can encode a kind of &#945;-carbonic anhydrase that is able to speed up the reversible conversion between CO2 and bicarbonate. In our design, engineered Pseudomonas aeruginosa PAO1 converse CO2 into bicarbonate, in order to deliver the carbon source in the biofilm more easily. Rhodopseudomonas palustris CGA009 take in the bicarbonate and transform it as CO2 for CBB cycle. The efficiency of the conversion in Rhodopseudomonas palustris WT is limited. Therefore, we introduce acaP into Rhodopseudomonas palustris CGA009 to realize overexpressing of the carbonic anhydrase, which enable the engineered Rhodopseudomonas palustris to converse bicarbonate into CO2 in a higher rate, accelerating the process of carbon fixation in Rhodopseudomonas palustris CGA009.
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