Revision as of 20:57, 14 August 2024

Yellow chromoprotein secreted by Saccharomyces cerevisiae and binded to cellulose

Description

Our project aims to create a fully bio-sourced and biodegradable insect trap. To achieve this, the base of our trap will be made of cellulose produced by a bacterium in a co-culture system. The other organism in this co-culture is Saccharomyces cerevisiae, a yeast that will secrete a yellow chromoprotein and a bioglue (BBa_K5143022). The adhesion of the yellow chromoprotein to the cellulose, which forms the base of our trap, will make it coloured, trapping insects that are specifically attracted to yellow.
To allow the secretion and the cellulose binding of the fwYellow, two elements are fused to the fwYellow gene :
1) Upstream of the fwYellow gene : Alpha-factor (BBa_K5143009), a yeast secretion signal allowing the excretion of the fused yellow protein out of the yeast cell.
2) Downstream of the fwYellow gene : Cellulose Binding Domain (BBa_K5143007), allowing the binding of the fused yellow protein to the cellulose.
Because yellow can attract many insects, the chromoprotein could easily be changed by cloning methods in order to change the colour of the trap and attract different targets.

The Yellow chromoprotein is secreted by Saccharomyces cerevisiae, then it binds to the cellulose to functionalize it.

Construction

This sequence was designed to be fully optimized for expression in Saccharomyces cerevisiae and for synthesis. This fragment was then cloned with the bioglue fragment (BBa_K5143022) in order to produce the final plasmid that was transformed into yeast cells and integrated into the yeast genome.

References

1. Gilbert, C. et al. Living materials with programmable functionalities grown from engineered microbial co-cultures. Nat. Mater. 20, 691–700 (2021).
2. Liljeruhm, J. et al. Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Journal of Biological Engineering 12, 8 (2018).

Sequence and Features