Difference between revisions of "Part:BBa K5143021"
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<partinfo>BBa_K5143021 short</partinfo> | <partinfo>BBa_K5143021 short</partinfo> | ||
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+ | <head> | ||
+ | <meta charset="UTF-8"> | ||
+ | <meta name="viewport" content="width=device-width, initial-scale=1.0"> | ||
+ | <title>Protein Description</title> | ||
+ | <style> | ||
+ | .image-container { | ||
+ | text-align: center; | ||
+ | margin: 20px auto; | ||
+ | border: 1px solid #000; | ||
+ | padding: 10px; | ||
+ | width: fit-content; | ||
+ | } | ||
+ | .image-container img { | ||
+ | width: 600px; | ||
+ | } | ||
+ | .image-caption { | ||
+ | text-align: center; | ||
+ | font-style: italic; | ||
+ | margin-top: 10px; | ||
+ | } | ||
+ | </style> | ||
+ | </head> | ||
+ | <body> | ||
+ | <h1>Description</h1> | ||
+ | <p> | ||
+ | Modifier | ||
+ | </p> | ||
+ | <p> | ||
+ | Modifier | ||
+ | </p> | ||
+ | <div class="image-container"> | ||
+ | <img src="https://static.igem.wiki/teams/5143/bba-k5143021-mruby-microscpoe.png" alt="mRuby AGA2"> | ||
+ | <div class="image-caption">Figure 1. Modifier</div> | ||
+ | </div> | ||
+ | <h2>Details of the System Function</h2> | ||
+ | <p> | ||
+ | Modifer | ||
+ | </p> | ||
+ | <h1>Construction</h1> | ||
+ | <p> | ||
+ | The gene encoding the chimeric mRuby2 protein fused to AGA2 and 6HIS-Tag has been optimized for synthesis and expression in <i>Saccharomyces cerevisiae</i>. We have placed this coding region downstream of the ADH1promoter to ensure strong constitutive expression. The entire construct has been synthesized. | ||
+ | <br> | ||
+ | This genetic construct has been cloned into the following plasmid backbone: <a href=""></a><br> | ||
+ | Resulting in the following integrative plasmid: | ||
+ | |||
+ | </p> | ||
+ | <h1>References</h1> | ||
+ | <p> | ||
+ | Modifier | ||
+ | </p> | ||
+ | </body> | ||
+ | </html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 16:42, 31 July 2024
Ruby fused with AGA2 under the control of the ADH1 promoter
Description
Modifier
Modifier
![mRuby AGA2](https://static.igem.wiki/teams/5143/bba-k5143021-mruby-microscpoe.png)
Figure 1. Modifier
Details of the System Function
Modifer
Construction
The gene encoding the chimeric mRuby2 protein fused to AGA2 and 6HIS-Tag has been optimized for synthesis and expression in Saccharomyces cerevisiae. We have placed this coding region downstream of the ADH1promoter to ensure strong constitutive expression. The entire construct has been synthesized.
This genetic construct has been cloned into the following plasmid backbone:
Resulting in the following integrative plasmid:
References
Modifier
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 180
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 830