Difference between revisions of "Part:BBa K5143001"
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The dragline protein MaSp1 containing numerous alanine and glycine residues tends to form considerable β-sheet and random coil secondary structure, which account for the crystalline fraction of cobwebs and confer inherently remarkable mechanical properties. The adhesion strenght of MaSp1 : 22.7 mJ/m².    </p>
 
The dragline protein MaSp1 containing numerous alanine and glycine residues tends to form considerable β-sheet and random coil secondary structure, which account for the crystalline fraction of cobwebs and confer inherently remarkable mechanical properties. The adhesion strenght of MaSp1 : 22.7 mJ/m².    </p>
 
      
 
      
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<img src="https://static.igem.wiki/teams/5143/bba-k5143001-masp1.png" width="400" alt="MaSp1">
 
     <h1>Construction</h1>
 
     <h1>Construction</h1>
 
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     <p>

Revision as of 10:48, 30 July 2024

Protein Description

Description

MaSp1 is a spider silk protein that can be used as a bioglue. The dragline protein MaSp1 containing numerous alanine and glycine residues tends to form considerable β-sheet and random coil secondary structure, which account for the crystalline fraction of cobwebs and confer inherently remarkable mechanical properties. The adhesion strenght of MaSp1 : 22.7 mJ/m².

MaSp1

Construction

We had MaSp1 synthesised and cloned with Cp19k and then cloned within the composite part: put compsoite part

References

Malay, A. D., Craig, H. C., Chen, J., Oktaviani, N. A. & Numata, K. Complexity of Spider Dragline Silk. Biomacromolecules 23, 1827–1840 (2022). Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk | PNAS. https://www-pnas-org.docelec.univ-lyon1.fr/doi/full/10.1073/pnas.2107065118. A bioinspired synthetic fused protein adhesive from barnacle cement and spider dragline for potential biomedical materials - PubMed. https://pubmed.ncbi.nlm.nih.gov/37776922/.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 191
    Illegal PstI site found at 494
    Illegal PstI site found at 587
    Illegal PstI site found at 593
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 191
    Illegal PstI site found at 494
    Illegal PstI site found at 587
    Illegal PstI site found at 593
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 22
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 191
    Illegal PstI site found at 494
    Illegal PstI site found at 587
    Illegal PstI site found at 593
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 191
    Illegal PstI site found at 494
    Illegal PstI site found at 587
    Illegal PstI site found at 593
  • 1000
    COMPATIBLE WITH RFC[1000]