Difference between revisions of "Part:BBa K5143001"
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− | Description | + | <html lang="en"> |
− | MaSp1 | + | <head> |
− | The dragline protein MaSp1 containing numerous alanine and glycine residues tends to form considerable β-sheet and random coil secondary structure, which account for the crystalline fraction of cobwebs and confer inherently remarkable mechanical properties. The adhesion strenght of MaSp1 : 22.7 mJ/m². | + | <meta charset="UTF-8"> |
− | + | <meta name="viewport" content="width=device-width, initial-scale=1.0"> | |
− | Construction | + | <title>Protein Description</title> |
− | We had MaSp1 synthesised and cloned with Cp19k and then cloned within the composite part: put compsoite part | + | </head> |
− | + | <body> | |
− | + | <h1>Description</h1> | |
− | References | + | <p> |
+ | MaSp1 is a spider silk protein that can be used as a bioglue. | ||
+ | The dragline protein MaSp1 containing numerous alanine and glycine residues tends to form considerable β-sheet and random coil secondary structure, which account for the crystalline fraction of cobwebs and confer inherently remarkable mechanical properties. The adhesion strenght of MaSp1 : 22.7 mJ/m². </p> | ||
+ | |||
+ | <img src=""> | ||
+ | <h1>Construction</h1> | ||
+ | <p> | ||
+ | We had MaSp1 synthesised and cloned with Cp19k and then cloned within the composite part: put compsoite part | ||
+ | |||
+ | </p> | ||
+ | <h1>References</h1> | ||
+ | <p> | ||
Malay, A. D., Craig, H. C., Chen, J., Oktaviani, N. A. & Numata, K. Complexity of Spider Dragline Silk. Biomacromolecules 23, 1827–1840 (2022). | Malay, A. D., Craig, H. C., Chen, J., Oktaviani, N. A. & Numata, K. Complexity of Spider Dragline Silk. Biomacromolecules 23, 1827–1840 (2022). | ||
Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk | PNAS. https://www-pnas-org.docelec.univ-lyon1.fr/doi/full/10.1073/pnas.2107065118. | Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk | PNAS. https://www-pnas-org.docelec.univ-lyon1.fr/doi/full/10.1073/pnas.2107065118. | ||
− | A bioinspired synthetic fused protein adhesive from barnacle cement and spider dragline for potential biomedical materials - PubMed. https://pubmed.ncbi.nlm.nih.gov/37776922/. | + | A bioinspired synthetic fused protein adhesive from barnacle cement and spider dragline for potential biomedical materials - PubMed. https://pubmed.ncbi.nlm.nih.gov/37776922/. </p> |
− | + | </body> | |
+ | </html> | ||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 10:45, 30 July 2024
Description
MaSp1 is a spider silk protein that can be used as a bioglue. The dragline protein MaSp1 containing numerous alanine and glycine residues tends to form considerable β-sheet and random coil secondary structure, which account for the crystalline fraction of cobwebs and confer inherently remarkable mechanical properties. The adhesion strenght of MaSp1 : 22.7 mJ/m².
Construction
We had MaSp1 synthesised and cloned with Cp19k and then cloned within the composite part: put compsoite part
References
Malay, A. D., Craig, H. C., Chen, J., Oktaviani, N. A. & Numata, K. Complexity of Spider Dragline Silk. Biomacromolecules 23, 1827–1840 (2022). Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk | PNAS. https://www-pnas-org.docelec.univ-lyon1.fr/doi/full/10.1073/pnas.2107065118. A bioinspired synthetic fused protein adhesive from barnacle cement and spider dragline for potential biomedical materials - PubMed. https://pubmed.ncbi.nlm.nih.gov/37776922/.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 191
Illegal PstI site found at 494
Illegal PstI site found at 587
Illegal PstI site found at 593 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 191
Illegal PstI site found at 494
Illegal PstI site found at 587
Illegal PstI site found at 593 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 22
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 191
Illegal PstI site found at 494
Illegal PstI site found at 587
Illegal PstI site found at 593 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 191
Illegal PstI site found at 494
Illegal PstI site found at 587
Illegal PstI site found at 593 - 1000COMPATIBLE WITH RFC[1000]